Human IFN-γ1b, premium grade 130-096-481 from Miltenyi Biotec

(1) Naïve Huntington’s disease microglia mount a normal response to inflammatory stimuli but display a partially impaired development of innate immune tolerance that can be counteracted by ganglioside GM1Journal of NeuroinflammationNovember 23, 2023Noam Steinberg, Danny Galleguillos, Asifa Zaidi, Melanie Horkey, Simonetta Sipionesystems (415ML/CF), recombinant human Interferon gamma1b (IFNγ1b) was purchased from Miltenyi Biotec (130-096-481). Exon1-mHTT97Q-eGFP and Exon1-wtHTT25Q-eGFP cloned in pcDNA3.1 plasmids were kindly provided by Dr.

(2) Mycobacterium tuberculosis impairs human memory CD4+ T cell recognition of M2 but not M1-like macrophagesiScienceSeptember 15, 2023Daniel P. Gail, Vinicius G. Suzart, Weinan Du, Avinaash Kaur Sandhu, Jessica Jarvela, Mary Nantongo, Ivan Mwebaza, Soumya Panigrahi, Michael L. Freeman, David H. Canaday, et al.300-03-50ug Recombinant Human M-CSF,Peprotech,Cat# 300-25-50ug "Human IFN-g1b, premium grade",Miltenyi Biotec,Cat# 130-096-484 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Human Memory

(3) The effect of serum origin on cytokines induced killer cell expansion and functionBMC ImmunologySeptember 1, 2023Zahra Jabbarpour, Seyed Sajjad Aghayan, Kobra Moradzadeh, Sasan Ghaffari, Naser Ahmadbeigisupplemented with 100 IU/ml penicillin/streptomycin (Bioidea, BI-1203), 1000 IU/ml IFN-γ (Miltenyi Biotech, 130-096-482), and 10%, 5%, and 2.5% of one of the following sera: FBS, hPL and HS on day 0. Hence, this study compares

(4) Nanostructured Microparticles Repolarize Macrophages and Induce Cell Death in an In Vitro Model of Tumour-Associated MacrophagesPharmaceuticsJuly 5, 2023Salma Al-Fityan, Britta Diesel, Thorben Fischer, Emmanuel Ampofo, Annika Schomisch, Vida Mashayekhi, Marc Schneider, Alexandra K. Kiemerdifferentiation, macrophages were left without polarization (M0) or polarized towards M1 by 20 ng/mL IFN-γ (130-096-484, Miltenyi Biotec) and 100 ng/mL LPS for 24 h. TAM-like macrophages were incubated in tumour cell-conditioned

(5) SAPrIm, a semi-automated protocol for mid-throughput immunopeptidomicsFrontiers in ImmunologyJune 2, 2023Terry C. C. Lim Kam Sian, Gabriel Goncalves, Joel R. Steele, Tima Shamekhi, Liesl Bramberger, Dongbin Jin, Mohammad Shahbazy, Anthony W. Purcell, Sri Ramarathinam, Stoyan Stoychev, et al.(Gibco) at 37°C with 5% CO2. Cells were treated with 50 IU of lyophilised human IFNγ (Miltenyi Biotec #130-096-484) for 48 h as per Goncalves et al. (2021). Cells were grown to 5e5, 5e6, 1e7 and 5e7 in three biological

(6) A druggable copper-signalling pathway that drives inflammationNatureMay 1, 2023Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD, et al.days. Subsequently, LPS (InvivoGen, tlrl-3pelps, 100 ng ml−1) and interferon-γ (IFNγ, Miltenyi Biotec, 130-096-484, 20 ng ml−1) were added to the medium for 24 h to generate aMDMs. CD4+ T cells were isolated from peripheral

(7) Characterization of Anti-Cancer Activities of Violacein: Actions on Tumor Cells and the Tumor MicroenvironmentFrontiers in OncologyMay 11, 2022Charlotte Dahlem, Shilpee Chanda, Jan Hemmer, Hanna S. Schymik, Michael Kohlstedt, Christoph Wittmann, Alexandra K. Kiemerin vitro: To obtain M1 polarization, media was supplemented with 20 ng/ml recombinant IFNγ (Miltenyi #130-096-484) and 100 ng/ml LPS (Ultrapure LPS from Escherichia coli K12 #tlrl-peklps), while either 20 ng/ml IL4

(8) IFN-γ Induces PD-L1 Expression in Primed Human BasophilsCellsFebruary 25, 2022Bonam SR, Chauvin C, Mathew MJ, Bayry J.stromal lymphopoietin (TSLP) (Catalogue: 1398-TS, lot: IDK0721051); Miltenyi Biotec: IFN-γ (Catalogue: 130-096-484, lot specific activity: ≥2 × 107 IU/mg, recombinant human granulocyte-macrophage colony-stimulating

(9) The Unitary Micro-Immunotherapy Medicine Interferon-γ (4 CH) Displays Similar Immunostimulatory and Immunomodulatory Effects than Those of Biologically Active Human Interferon-γ on Various Cell TypesInternational Journal of Molecular SciencesFebruary 19, 2022Camille Jacques, Mathias Chatelais, Karim Fekir, Adrien Brulefert, Ilaria Florisadding IFN-γ (4 CH) or vehicle to the medium. Commercial human recombinant IFN-γ (20 ng/mL) (# ref 130-096-481, Miltenyi Biotec B.V. and Co. KG, Bergisch Gladbach, Germany) was used as a positive control and run

(10) Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19ImmunityNovember 9, 2021Krämer B, Knoll R, Bonaguro L, ToVinh M, Raabe J, Astaburuaga-García R, Schulte-Schrepping J, Kaiser KM, Rieke GJ, Bischoff J, et al.11340064 Amphiregulin,PeproTech,Cat# 100-55B TNFa,Immunotools,Cat# 11343015 Human IFN-g1b premium grade,Miltenyi Biotec,Cat# 130-096-481 Antibiotic-Antimyotic,GIBCO Life,Cat# 15240-062 Human Serum AB Plasma,Sigma,Cat# H3667-100ml Fetal bovine serum

(11) Dysregulation of cholesterol homeostasis in human lung cancer tissue and tumour-associated macrophageseBioMedicineOctober 1, 2021Jessica HoppstÃ¤dter, Anna Dembek, Marcus HÃ¶ring, Hanna S. Schymik, Charlotte Dahlem, Afnan Sultan, Natalie Wirth, Salma Al-Fityan, Britta Diesel, Gilles Gasparoni, et al.for another 24 hours. To this end, MDM medium was supplemented with 20 ng/ml IFNγ (Miltenyi Biotec, #130-096-484) and 100 ng/ml LPS (Ultrapure LPS from Escherichia coli K12 #tlrl-peklps, Invivogen, San Diego, CA,

(12) Genetic Predisposition to the Mortality in Septic Shock Patients: From GWAS to the Identification of a Regulatory Variant Modulating the Activity of a CISH EnhancerInternational Journal of Molecular SciencesMay 29, 2021Florian Rosier, Audrey Brisebarre, Claire Dupuis, Sabrina Baaklini, Denis Puthier, Christine Brun, Lydie C. Pradel, Pascal Rihet, Didier PayenFor cell stimulation, 106 K562 cells were incubated with IFN-γ (Miltenyi, Bergisch Gladbach, Germany, 130-096-484) at 100 ng/mL for 6 h or LPS (Sigma, St. Louis, MO, USA, L9143) at 100 ng/mL for 24 h. 4.3. Single

(13) IFNγ Modulates the Immunopeptidome of Triple Negative Breast Cancer Cells by Enhancing and Diversifying Antigen Processing and PresentationFrontiers in ImmunologyApril 22, 2021Gabriel Goncalves, Kerry A. Mullan, Divya Duscharla, Rochelle Ayala, Nathan P. Croft, Pouya Faridi, Anthony W. Purcell37°C with 5% CO2. Cells were titrated at 50,100 and 200 IU of lyophilized human IFNγ (Miltenyi Biotec #130-096-484) for 48 h before being examined at a time course across 16, 24, 48-h intervals. IFNγ treated cells were

(14) Coding and non-coding roles of MOCCI (C15ORF48) coordinate to regulate host inflammation and immunityNature communicationsApril 9, 2021Lee CQE, Kerouanton B, Chothani S, Zhang S, Chen Y, Mantri CK, Hock DH, Lim R, Nadkarni R, Huynh VT, et al.macrophages in media containing 100 ng/mL LPS (InvivoGen tlrl-3pelps) and 20 ng/mL IFNg (Miltenyi Biotec 130-096-481). After 8 h, 1 ng/mL IL-1β or water were added into the appropriate wells while still in the presence

(15) Stromal-like Wilms tumor cells induce human Natural Killer cell degranulation and display immunomodulatory properties towards NK cellsOncoimmunologyMarch 8, 2021Cantoni C, Serra M, Parisi E, Azzarone B, Sementa AR, Nasto LA, Moretta L, Candiano G, Bottino C, Ghiggeri GM, et al.experiments, str-WT cells were cultured for 72 h in the presence of 20 ng/ml IFN-γ (Miltenyi Biotec, 130–096-482) or for 48 h with 20 ng/ml IL-1β (Miltenyi Biotec, 130–093-897) and 10 ng/ml TNF-α (Miltenyi Biotec,

(16) The HSP GRP94 interacts with macrophage intracellular complement C3 and impacts M2 profile during ER stressCell death & diseaseJanuary 22, 2021Chaumonnot K, Masson S, Sikner H, Bouchard A, Baverel V, Bellaye PS, Collin B, Garrido C, Kohli E.Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days, then respectively activated with 20 ng/ml of IFNγ (130-096-484, Miltenyi Biotec) and 100 ng/ml LPS (L2887, Sigma-Aldrich) or IL-4 (130-093-922, Miltenyi Biotec) and

(17) Thioholgamide A, a New Anti-Proliferative Anti-Tumor Agent, Modulates Macrophage Polarization and MetabolismCancers (Basel)May 19, 2020Charlotte Dahlem et al.(HMDMs) in vitro, the differentiation media was supplemented with 20 ng/mL recombinant IFNγ (Miltenyi #130-096-484) and 100 ng/mL LPS (Ultrapure LPS from Escherichia coli K12 #tlrl-peklps) for M1 polarization; either

(18) Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro CultureCellsMarch 23, 2020Daum R, Visser D, Wild C, Kutuzova L, Schneider M, Lorenz G, Weiss M, Hinderer S, Stock UA, Seifert M, et al.were used as negative control. To induce the polarization into the M1 phenotype, 20 ng/mL of IFNγ (130-096-486, Miltenyi Biotec) and 100 ng/mL of LPS were added to the medium of M0 macrophages. After two days of

(19) Simulating Inflammation in a Wound Microenvironment Using a Dermal Wound-on-a-Chip ModelAdvanced healthcare materialsJanuary 1, 2019Biglari S, Le TYL, Tan RP, Wise SG, Zambon A, Codolo G, De Bernard M, Warkiani M, Schindeler A, Naficy S, et al.InvivoGen tlrl-pb5lps) with final concentration of 100 ng/mL and Interferon gamma (IFN-) (MILTENYI, 130-096-481) with final concentration of 20 ng/mL for at least 24 hours to be polarized to M1 macrophages. In order

(20) IL-34 and CSF-1 display an equivalent macrophage differentiation ability but a different polarization potentialScientific reportsJanuary 10, 2018Boulakirba S, Pfeifer A, Mhaidly R, Obba S, Goulard M, Schmitt T, Chaintreuil P, Calleja A, Furstoss N, Orange F, et al.lipopolysaccharide (LPS-EK Ultrapure, Invivogen, tlrl-peklps) and 20 ng/mL interferon gamma (Miltenyi, 130-096-484) were added after 7 days of differentiation for two days to polarize into M1-macrophages. M2-macrophages

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Human IFN-γ1b, premium grade from Miltenyi Biotec

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Miltenyi Biotec

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Human IFN-γ1b, premium grade from Miltenyi Biotec

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